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Hydrophobic interactions are important for the Vif-CBFβ interaction. (A) Strategy used to test Vif-CBFβ complex stability under various conditions. Vif residues 1 to 192 and CBFβ140-His were expressed in E. coli and purified by nickel beads via a 6× His tag in CBFβ. (B to D) Vif-CBFβ complex stability under conditions with various pHs (B), salt (NaCl) concentrations (C), and detergent (Triton X-100) concentrations (D).
