FIG 4.

Hydrophobic residues in HIV-1 Vif are important for CBFβ binding. (A) Vif mutants used in this study. (B and C) Coimmunoprecipitation with anti-Myc matrix to check for CBFβ-Vif interactions. WT and mutant Vif-HA expression vectors were cotransfected with the CBFβ-Myc expression vector into 293T cells; 36 h later, the cells were harvested and the cell lysates were analyzed by Western blotting (B) or coimmunoprecipitation analysis with an anti-Myc matrix, followed by Western blotting with the indicated antibodies (C). (D) Coimmunoprecipitation of EloB with WT and mutant Vif-HA. IP, immunoprecipitation.