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. 2014 Mar;88(5):2555–2563. doi: 10.1128/JVI.03604-13

FIG 6.

FIG 6

Mutation of hydrophobic residues in Vif to Ser reduced the interaction between mutant Vif and CBFβ. (A) Vif mutants (W5S, W21S, W38S, W89S, F112S, and F115S) used in this study. (B) Vif-HA WT or mutant expression vectors and the CBFβ-Myc expression vector were cotransfected into 293T cells; the cells were harvested after 36 h. Coimmunoprecipitation of CBFβ-Myc, Cul5, or EloB with Vif-HA was analyzed by Western blotting. (C) Vif-HA WT or mutant expression vectors and the A3G-V5 expression vector were cotransfected into 293T cells. Coimmunoprecipitation of A3G-V5 with Vif-HA was analyzed by Western blotting. Vif-HA mutant (or WT) plasmids and A3G-V5 plasmids were cotransfected into HEK 293T cells; the cells were harvested after 36 h. Coimmunoprecipitation with anti-HA matrix was used to detect the interaction between Vif mutants and A3G.