FIG 3.

Effect of mutations in vdUTPase or the kinase-dead mutation in Us3 on viral replication in the CNS of mice following intracranial inoculation. (A to C) Fifteen (A and B) or twenty-five (C) 3-week-old female mice were infected intracranially with YK750 (ΔvdUTPase) (A), YK751 (vdUTPaseS187A) (A and C), YK752 (vdUTPaseΔ/SA-repair) (A and C), YK511 (Us3K220M) (B), YK513 (Us3K220M-repair) (B), YK753 (vdUTPaseS187D) (C), or YK754 (vdUTPaseS187D-repair) (C). At 3 days postinfection, the brains of infected mice were harvested, and virus titers were assayed. Each data point is the virus titer in the brain of one mouse. The horizontal bars indicate the mean for each group. P represents the statistical significance value according to the two-tailed Student t test. n.s., not significant.