TABLE 1.
Primer sequences for the construction of recombinant viruses
| Mutation | Orientationa | Sequence (5′–3′) |
|---|---|---|
| ICP22S5A/S22A | F | CACTGTGGTCCTCCGGGACGTTTTCTGGATGGCCGACATTGCCCCAGGCGCTTTTGCGCCTTGTGTAAAAGCGCGGCGTCCCGCTCTCCGAGCCAGGATGACGACGATAAGTAGGG |
| R | GGCGGGAAGGGCGCTTGCGCTTGCGCGTGCCCAGGGGCGGGGCTCGGAGAGCGGGACGCCGCGCTTTTACACAAGGCGCAAAAGCGCCTGGGGCCAACCAATTAACCAATTCTGATTAG | |
| ICP22T162A/S167A | F | GCGGGTCCGGTCTACCCGGGAAACGCAGCCCCGGGCCCCCGCCCCGTCGGCCCCAGCCCCAAATGCAATGAGGATGACGACGATAAGTAGGG |
| R | TCTGGGCCTGGCGCACCGAGCGCCGTAGCATTGCATTTGGGGCTGGGGCCGACGGGGCGGGGGCCCGGGGCAACCAATTAACCAATTCTGATTAG | |
| ΔICP22 | F | GCGGGGGGAAGCCACTGTGGTCCTCCGGGACGTTTTCTGGGTCCGGTCGCCCCGACCCCCAGGATGACGACGATAAGTAGGG |
| R | TTTTATTTGGGGACATACAAGGGGGTCGGGGCGACCGGAC-CCAGAAAACGTCCCGGAGGACAACCAATTAACCAATTCTGATTAG | |
| ΔICP22-repair | F | GCGGGGGGAAGCCACTGTGGTCCTCCGGGACGTTTTCTGGATGGCCGACATTTCCCCAGGCGC |
| R | CTTTTATTTGGGGACATACAAGGGGGTCGGGGCGACCGGACTCACGGCCGGAGAAACGTGT | |
| UL51T190A | F | GCTTGGGGTGACCGAGGCGCCCTCCTTGGGGCACCCCCACGCACCGCCCCCGGAGGTTACAGGATGACGACGATAAGTAGGG |
| R | CGTTTCGGGCGGCAGGCGCCAGCGTAACCTCCGGGGGCGGTGCGTGGGGGTGCCCCAAGGCAACCAATTAACCAATTCTGATTAG | |
| UL51T190A-repair | F | GCTTGGGGTGACCGAGGCGCCCTCCTTGGGGCACCCCCACACGCCGCCCCCGGAGGTTACAGGATGACGACGATAAGTAGGG |
| R | CGTTTCGGGCGGCAGGCGCCAGCGTAACCTCCGGGGGCGGCGTGTGGGGGTGCCCCAAGGCAACCAATTAACCAATTCTGATTAG |
a
F, forward; R, reverse.