FIG 5.

DRB3 interacts with DCL3 and AGO4 in distinct subnuclear bodies. BiFC analysis of the indicated DCL, DRB, and AGO proteins in N. benthamiana epidermal cells was performed. Constructs expressing proteins fused to the N- or C-terminal portion of YFP were delivered by agroinfiltration to N. benthamiana leaves. Cells were photographed at 36 h postinfiltration using a confocal laser scanning microscope. RFP-histone 2B (RFP-H2B) and RFP-fibrillarin were used as markers for the nucleus and nucleolus, respectively. Protein combinations are indicated above each photograph. The photographs in panel A show lower-magnification views (×20), while panel B shows high-magnification views of nuclei (×100).