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. 2014 Mar;88(5):2748–2762. doi: 10.1128/JVI.03588-13

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FIG 2 — Expression of the HCV NS proteins by baculovirus vectors. (a) Schematic of the various baculovirus constructs generated. (b) Western blot analysis of NS proteins after transduction of Huh7.5 (top) and HepG2 (bottom three panels) with the baculovirus constructs. Cells were transduced with the following baculovirus vectors: FBM(NS5A^GFP) (lane 2), FBM(NS4B-5A^GFP) (lane 3), FBM(NS4A-5A^GFP) (lane 4), FBM(NS3-5A^GFP) (lane 5), FBM(NS3-5B^GFP) (lane 6), FBM(NS4B-5A^GFP) plus FBM(NS3) (lane 7), FBM(NS4B-5A^GFP) plus FBM(NS3-4A) (lane 8), FBM(NS4A-5A^GFP) plus FBM(NS3) (lane 9), FBM(NS4B) (lane 10), FBM(NS4A-4B) (lane 11), FBM(NS3-4B) (lane 12), FBM(NS4A-4B) plus FBM(NS3) (lane 13), FBM(NS3) (lane 14), and FBM(NS3-4A) (lane 15). For comparison, cells were transduced with a baculovirus that expressed lacZ (lane 1). *, bands corresponding to the expected size of mature HCV proteins; #, bands corresponding to the size of uncleaved polyprotein products.