Skip to main content
. 2014 Mar;88(5):2748–2762. doi: 10.1128/JVI.03588-13

FIG 5.

FIG 5

Intragenomic complementation of defective HCV RNA replication. (a) Schematic representation of bicistronic replicon constructs with the first cistron encoding a Renilla luciferase-FMDV2A reporter fusion protein linked to NS4B (Rep_R2NS4B/3-5BFLAG), NS5A (Rep_R2NS5AV5/3-5BFLAG), and NS3-NS5A (Rep_R2NS3-5AV5/3-5BFLAG). (b) Transient replication assays in Huh7.5 cells. wt, S2208I, and G1911A refer to the NS3-5B polyprotein expressed in the second cistron for each construct; wt denotes wt versions of NS5A and NS4B, S2208I indicates the mutation in NS5A, and G1911A denotes the mutation in NS4B. The ΔGDD construct expresses a nontagged version of NS5A in the first cistron and NS3-5B containing a defective HCV RNA polymerase in the second cistron. Luciferase activity was monitored over 72 h and normalized to that at the 4-h time point (mean ± SD; n = 2).