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Generation of a stable Vero-cNectin-4 cell line. Vero cells were transduced with lentiviral vectors carrying the canine nectin-4 gene. The gene was designed to express a protein with the HA tag fused N terminally. IF (A to C) and flow cytometry (D) of Vero cells, Vero-cSLAM cells (SLAM also bears an additional N-terminal HA tag), and Vero-cNectin-4 cells were performed as described in Fig. 1H, with the exception of the primary antibody used (anti-HA 16B12).
