TABLE 1.
Prefusion F protein stability of CDV
CDV | Temp of conformation switch (°C)a |
|
---|---|---|
No drug | +3g | |
F-wt | 58–60 | 65–67 |
F-L326A | 50–52 | 60–62 |
F-L372G | 44–46 | 51–53 |
F-L372A | 51–53 | 60–62 |
F-P331A | 50–52 | 60–62 |
F-S332A | 50–52 | 60–62 |
F-V447T | 51–53 | 61–63 |
Determined by detecting the temperature at which 50% of the F population was bound by the antiprefusion MAb and the other 50% was bound by the antitrigger MAb.