FIG 5.

Phosphorylation and dephosphorylation of HBc ARD peptides can influence DNA annealing activity. (A) Amino acid sequences of different HBc ARD peptides phosphorylated at different positions. (B) Characterization of HBc ARD peptides by Tricine SDS-PAGE. Peptides were stained with Green Angel. (C) Labeled Tar(+) and cold Tar(−) were incubated with 25 and 50 nM phosphorylated HBc ARD peptides at 37°C for 5 min. (D) Rescue of the reduced DNA annealing activity of HBc ARD peptides by dephosphorylation treatment with lambda phosphatase.