FIG 1.
PRRSV induces PGE2 production in vitro and in vivo. (A) Relationship of pig temperature at day 6 after HP-PRRSV strain JXnw06 infection and pig survival (in days). (B) PAMs were incubated with HP-PRRSV JXnw06 or type 2 PRRSV strain CH-1a at an MOI of 2, and then cell culture supernatants were collected at 0, 4, 8, 12, 24, and 36 h postincubation for PGE2 analysis using an ELISA kit. (C and D) Pigs were infected intranasally with 1 ml (105.2 TCID50s) HP-PRRSV strain JXnw06; plasma was collected at days 0, 3, and 7 postinfection and treated with indomethacin, and BALF was collected when the pigs were dying. PGE2 in plasma (C) and BALF (D) was quantified by ELISA. Data are means ± SEMs from three independent experiments. ctrl, control. Differences were evaluated by Student's t test (*, P < 0.05; **, P < 0.01).