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Primers for truncated sequence of COX-1 promotera
| Primer name | Sequence |
|---|---|
| +71-smaI | TCCCCCGGGGGTGCGGGATACAGACTGC |
| −215/71-luc | GGGGTACCTTTCCTATCTTCCTAATGGGC |
| −111/71-luc | GGGGTACCTAATTAATAAAATGTCTTGGCC |
| −37/71-luc | GGGGTACCGAGAGGTGGAGCCAGGGA |
Nucleotide +1 represents the site of transcription initiation of the COX-1 promoter, and a truncated COX-1 was cloned at the KpnI and SmaI sites in the pGL3-basic luciferase reporter vector. Underlining shows the KpnI enzyme site.
