TABLE 1.
V/P gene and SH gene sequences of rMuVΔSHΔV P10 single plaque-purified virusesa
| Virus | V protein deletion | NP GE or V/P GS mutation | V/P editing site mutation | SH ORF deletion |
|---|---|---|---|---|
| SP-1 | Yes | 1913 C-T | 2445 G-A | Yes |
| SP-2 | Yes | 1913 C-T | 2445 G-A | Yes |
| SP-3 | Yes | 1913 C-T | 2445 G-A | Yes |
| SP-4 | Yes | 1913 C-T | 2445 G-A | Yes |
| SP-5 | Yes | 1913 C-T | 1578 A-C | Yes |
| SP-6 | Yes | 1913 C-T | 2445 G-A | Yes |
| SP-7 | Yes | 1913 C-T | 2445 G-A | Yes |
| SP-8 | Yes | 1913 C-T | 2445 G-A | Yes |
| SP-9 | Yes | 1913 C-T | 2445 G-A | Yes |
| SP-10 | Yes | 1913 C-T | 2445 G-A | Yes |
a
rMuVΔSHΔV was passed continuously in Vero cells for 10 passages. Ten plaques were randomly obtained from rMuVΔSHΔV at passage 10 and grown in Vero cells (SP-1 to SP-10). The V/P and SH gene regions of SP-1 to SP-10 were sequenced. Mutations found in these regions are shown.