TABLE 1.
PrPSc detection in tissues of WT PRNP goats orally challenged with scrapie agent and sequentially killed
| Organa | 30 dpi |
90 dpi |
180 dpi |
360 dpi |
540 dpi |
940 dpi |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. of positive individuals/no. of animals testedb | PrPSc label intensityc | No. of positive individuals/no. of animals testedb | PrPSc label intensityc | No. of positive individuals/no. of animals testedb | PrPSc label intensityc | No. of positive individuals/no. of animals testedb | PrPSc label intensityc | No. of positive individuals/no. of animals testedb | PrPSc label intensityc | No. of positive individuals/no. of animals testedb | PrPSc label intensityc | |
| Obex | 0/3 | − | 0/4 | − | 0/3 | − | 0/3 | − | 0/4 | − | 4/4 | +/+++ |
| Spinal cord (cervical) | ND | ND | ND | 0/3 | − | 0/4 | − | 4/4 | +/++ | |||
| Spinal cord (thoracic) | ND | ND | ND | 0/3 | − | 0/4 | − | 4/4 | +/+++ | |||
| Spinal cord (lumbar) | ND | ND | ND | 0/3 | − | 0/4 | − | 4/4 | +/+++ | |||
| Tonsil | 0/3 | − | 0/4 | − | 0/3 | − | 1/3 (a) | ++ | 2/4 (a, b) | +/+++ | 4/4 | +++ |
| Parotid LN | 0/3 | − | 0/4 | − | 0/3 | − | 1/3 (a) | + | 3/4 (a to c) | ++/+++ | 4/4 | +++ |
| Retropharyngeal LN | 0/3 | − | 0/4 | − | 0/3 | − | 2/3 (a, b) | +/++ | 3/4 (a to c) | ++/+++ | 4/4 | ++++ |
| Spleen | 0/3 | − | 0/4 | − | 0/3 | − | 0/3 | − | 1/4 (a) | + | 4/4 | +/+++ |
| Duodenal PP | 0/3 | − | 0/4 | − | 0/3 | − | 3/3 | +++ | 4/4 | +++ | 4/4 | +++ |
| Jejunal PP | 0/3 | − | 0/4 | − | 2/3 (a, b) | + | 3/3 | +++ | 4/4 | +++ | 4/4 | +++ |
| Ileal PP | 0/3 | − | 0/4 | − | 2/3 (a, b) | +/++ | 3/3 | +++ | 4/4 | +++ | 4/4 | +++ |
| Cecal PP | 0/3 | − | 0/4 | − | 0/3 | − | 1/3 (a) | ++ | 4/4 | ++/+++ | 4/4 | ++/+++ |
| Jejunal MLN | 0/3 | − | 0/4 | − | 2/3 (a, b) | + | 3/3 | +/+++ | 4/4 | +++ | 4/4 | +++ |
| Ileal MLN | 0/3 | − | 0/4 | − | 1/3 (a) | + | 3/3 | +++ | 4/4 | ++++ | 4/4 | +++ |
| Mediastinal LN | 0/3 | − | 0/4 | − | 0/3 | − | 1/3 (a) | + | 2/4 (a, b) | +/+++ | 4/4 | +++ |
| Prescapular LN | 0/3 | − | 0/4 | − | 0/3 | − | 1/3 (a) | + | 0/4 | − | 4/4 | +/+++ |
| Retrohepatic LN | 0/3 | − | 0/4 | − | 0/3 | − | 1/3 (a) | ++ | 4/4 | ++/+++ | 4/4 | ++/+++ |
| Duodenum (ENS) | 0/3 | − | 0/4 | − | 0/3 | − | 0/3 | − | 4/4 | +/++ | 4/4 | +/++ |
| Jejunum (ENS) | 0/3 | − | 0/4 | − | 0/3 | − | 1/3 (a) | + | 4/4 | +/++ | 4/4 | +/++ |
| Ileum (ENS) | 0/3 | − | 0/4 | − | 0/3 | − | 1/3 (a) | + | 4/4 | +/++ | 4/4 | +/++ |
| Cecum (ENS) | 0/3 | − | 0/4 | − | 0/3 | − | 0/3 | − | 3/4 (a to c) | +/++ | 4/4 | +/++ |
| Colon (ENS) | 0/3 | − | 0/4 | − | 0/3 | − | 0/3 | − | 3/4 (a to c) | +/++ | 4/4 | +/++ |
| Sciatic nerve | 0/3 | − | 0/4 | − | 0/3 | − | 0/3 | − | 0/4 | − | 4/4 | + |
| Brachial nerve | 0/3 | − | 0/3 | − | 0/3 | − | 0/3 | − | 0/4 | − | 4/4 | + |
| External ocular muscle | 0/3 | − | 0/3 | − | 0/3 | − | 0/3 | − | 0/4 | − | 4/4 | + |
a
For the spinal cord, cervical (C3-C4), thoracic (Th7-Th8), and lumbar (L3-L4) segments were analyzed. LN, lymph nodes; PP, Peyer's patches; MLN, mesenteric lymph nodes; ENS, enteric nervous system.
b
At each time point, the positive goats are identified arbitrarily by the letters a to d. ND, no data.
c
PrPSc labeling intensities are indicated as follows (as previously described [26]): −, negative; +, minimal to slight; ++, moderate; and +++, strong.