FIG 3.

Detection of budding and pestivirus particles in the ER compartment. MDBK cells infected with pestivirus Giraffe-1 were analyzed by EM. ER, endoplasmic reticulum; G, Golgi complex; M, mitochondrion. (A) Virion budding into the ER lumen at 15 h p.i. The white frame indicates the section depicted in panel B. Bar = 200 nm. (B) Detailed view of double lipid membrane protrusion of arising virion. The orthoslice picture was obtained by electron tomography. Bar = 50 nm. (C) Virions in separate ER lumina (arrows) at 12 h p.i. Bar = 200 nm.