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Double-immunofluorescence staining of CA XIV and anti-SERCA2 in adult cardiomyocytes of mouse. (A-D) Cardiomyocytes of wild-type mouse. (A) Incubation with preimmune serum and anti-rabbit IgG/FITC. (B) Anti-mouse CA XIV/FITC. (C) Anti-mouse SERCA2/TRITC. (D) Merge of B and C. (E-G) Cardiomyocytes of CA XIV KO mouse. (E) Anti-mouse CA XIV/FITC. (F) Anti-mouse SERCA2/TRITC. (G) Merge of E and F. Confocal laser scanning microscopy.
