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. 2014 Mar 18;34(2):e00099. doi: 10.1042/BSR20130096

Figure 3. BMH2 but not BMH1 acts at the promoters of ribosome biogenesis genes to attenuate their repression following rapamycin treatment.

Figure 3

(A) Quantitative RT-PCR was used to determine the transcriptional effect of treating the indicated yeast strain (WT, bmh1 and bmh2) with rapamycin. Each data point represents the abundance of NMD3, NSA2 and NOG2 RNA in rapamycin-treated cells compared with vehicle-treated cells, relative to the RNA levels of TUB2. Error bars indicate the standard error of the mean (n=3). (B) ChIP followed by quantitative PCR was used to determine the extent to which Bmh1 and Bmh2 bind to the promoters of NMD3, NSA2 and NOG2. Each data point represents the fold enrichment of the indicated promoter after the immunoprecipitation compared with the whole cell extract, relative to the enrichment of the promoter of TUB2. Error bars indicate the standard error of the mean (n=4).