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. 2014 Mar 18;34(2):e00099. doi: 10.1042/BSR20130096

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© 2014 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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Quantitative RT-PCR was used to determine the transcriptional effect of deleting one 14-3-3 gene in (A) untreated cells and (B) cells treated with rapamycin. Each data point represents the abundance of BMH1 or BMH2 RNA in the indicated mutant cells compared with WT cells (A) or in rapamycin-treated cells compared with vehicle-treated cells, relative to the levels of TUB2. Error bars indicate the standard error of the mean (n=3).