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. 2014 Mar 18;34(2):e00098. doi: 10.1042/BSR20130127

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© 2014 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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(A) The schematics of DHCR24-V5, Δ160 DHCR24-V5 and Δ240 DHCR24-V5 shown in relation to the putative TMD and other lower scoring putative TMDs (c, d, e) and Insig-myc. (B) CHO-7 cells were transfected with either 4 μg DHCR24-V5 (WT), Δ160 DHCR24-V5 (Δ160) or Δ240 DHCR24-V5 (Δ240), and co-transfected with 1 μg Insig-1-myc in a 10-cm dish for 24 h. Cell lysate was fractionated, and the nuclear (N), membrane (M) and cytoplasmic (C) fractions were separated by SDS–PAGE (10% gel) and immunoblotted with antibodies against V5 (DHCR24) and myc (Insig). Data from n=3 experiments.