Table 2. Kinetic parameters of PKN1, PKN2 and PKN3.
Means±S.D. (n ≥ 2) using off-chip mobility shift assay for full-length, FLAG-tagged PKN1, PKN2 and PKN3 purified from HEK-293 cells as described in the Experimental section.*
| Kinase | kcat (s−1) | KM,ATP (mM) | Kia (μM) | kcat/KM,ATP (M−1 s−1) | KM,Pep (μM) | kcat/KM,Pep (M−1 s−1) | |
|---|---|---|---|---|---|---|---|
| PKN1 | 0 μM AA | 0.7±0.1 | 0.24±0.02 | 230±10 | 3×103 | 13±1 | 5×104 |
| 100 μM AA | 13±6 | 0.18±0.05 | 19±10 | 7.2×104 | 8±3 | 2×106 | |
| PKN2 | 0 μM AA | 0.10±0.01 | 0.06±0.02 | 50±8 | 2×103 | 1.8±0.8 | 5.6×104 |
| 100 μM AA | 0.9±0.1 | 0.010±0.003 | 2±1 | 9×104 | 1.3±0.3 | 7×105 | |
| PKN3 | 0 μM AA | 1.0±0.1 | 0.22±0.03 | 10±9 | 5×103 | 31±5 | 3×104 |
| 5 μM AA | 1.1±0.1 | 0.067±0.005 | 14±3 | 1.6×104 | 7.9±0.5 | 1.4×105 |
*AA, arachidonic acid.