Figure 1.

Gel detection of RPA products. Amplified products were detected using gel electrophoresis stained with ethidium bromide. Using DNA extracted from oocysts spiked into PBS, RPA products from as few as 10³ oocysts/mL PBS (A) are visible on the gel. Using DNA extracted from oocysts spiked into uninfected stool samples from healthy volunteers, RPA products from as few as 10⁴ oocysts/mL stool (B) are visible on the gel.