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. 2014 Mar 6;176(1):37–48. doi: 10.1111/cei.12243

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© 2013 The Authors. Clinical and Experimental Immunology published by John Wiley & Sons Ltd on behalf of British Society for Immunology, Clinical and Experimental Immunology

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effect of CD8⁺ tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL)^– cells on suppression. (a) Flow cytometry gate used for analysis. Spleen cells were cultured with antigen-presenting cells (APC) treated with transforming growth factor (TGF)-β2 and interphotoreceptor retinoid-binding protein (IRBP). Seven days later the CD8⁺ T cells were enriched by magnetic beads and single-stained for CD8 (top panel). Lower panel: an aliquot of enriched CD8⁺ T cells was stained with TRAIL phycoerythrin (PE) (abscissa) and forkhead box protein 3 (FoxP3) (fluorescein isothiocyanate (FITC) (ordinate). (b) Local adoptive transfer assay using CD8⁺TRAIL^– T cells. The experiment was performed twice with similar results. *Significant difference (P ≤ 0·05).