Table 2.

Experimental design matrix employed for K. lactis cells permeabilization and average β-gal activity of permeabilized cells.

Run	Independent variable			Response
	x1	x2	x3	β-gal activity (mmol L−1 oNP min−1 g−1)
1	−1	−1	−1	1,365
2	−1	−1	+1	786.2
3	−1	+1	−1	1,468
4	−1	+1	+1	127.2
5	+1	−1	−1	3,197
6	+1	−1	+1	6.00
7	+1	+1	−1	985.3
8	+1	+1	+1	0.00
9	−1.68	0	0	146.9
10	+1.68	0	0	357.0
11	0	−1.68	0	975.2
12	0	+1.68	0	115.4
13	0	0	−1.68	1,311
14	0	0	+1.68	8.48
15	0	0	0	187.1
16	0	0	0	163.1
17	0	0	0	183.5
18	0	0	0	135.1
19	0	0	0	208.2