Table 1.
Strain | Specie | MIC HgCl2 (μg/mL) | Volatilization assay | merA gene amplification* | merA gene hybridization | merR gene amplification | Antibiotic resistance pattern |
---|---|---|---|---|---|---|---|
6610 | A. hydrophila | 400 | + | + | ND | − | TET |
6632 | A. hydrophila | 200 | + | + | + | + | S |
6479 | A. hydrophila | 300 | + | + | ND | + | TET, NAL, TMP, STR |
6698 | A. hydrophila | 200 | + | + | ND | − | S |
6689 | A. hydrophila | 200 | + | − | + | + | S |
6587 | A. hydrophila | 200 | + | + | ND | + | TET, NAL, TMP, STR |
6492 | A. hydrophila | 200 | + | + | ND | − | S |
6376 | A. hydrophila | 25 | + | − | + | − | TET |
5794 | A. hydrophila | 200 | + | − | + | + | TET, TMP, STR |
3510 | A. hydrophila | 200 | + | + | ND | + | S |
3611 | A. hydrophila | 600 | + | + | ND | + | TET |
6640 | A. aquariorum | 300 | + | − | + | + | TET, NAL, TMP |
6336 | A. aquariorum | 200 | + | − | + | − | TET, NAL, TMP |
3430-1 | A. aquariorum | 300 | + | + | ND | + | TET, TMP, STR |
6597 | A. caviae | 25 | + | + | ND | + | TET, TMP |
6626 | A. caviae | 300 | + | + | ND | − | TET, TMP |
6425 | A. caviae | 200 | + | + | ND | + | TET, TMP |
6436 | A. caviae | 25 | + | + | ND | − | TET, TMP |
6661 | A. caviae | 25 | + | − | + | + | TMP |
6582 | A. caviae | 25 | + | − | + | + | TET, TMP |
6548 | A. caviae | 25 | + | − | + | − | TMP |
5881 | A. caviae | 200 | + | − | + | + | TMP, STR |
5919 | A. caviae | 25 | + | − | + | − | TET, TMP |
3817 | A. caviae | 300 | + | − | + | + | TET, NAL, TMP, STR |
6654 | A. veronii | 200 | + | − | + | + | S |
5869 | A. veronii | 200 | + | + | ND | − | TET, NAL, TMP |
5933 | A. veronii | 300 | + | + | ND | + | TET, TM, CAM |
3925 | A. media | 25 | + | − | + | − | TMP, STR |
TET, Tetracycline; TMP, Trimethoprim; STR, streptomycin; NAL, nalidixic acid; CAF, Chloramphenicol; ND, not done; S, sensitive to all antibiotics tested.
Amplified with primers MerA1F/MerA1R and MerA1/MerA2.