Table 2.

Isolates of Methylobacterium spp. and other genera from different plant hosts, identified by partial 16S rRNA gene sequencing, and used to evaluate primers specificity.

Isolate	Host plant	Genes
		mxaF	acdS	Patatin	crtI	phoU	sss	recA
Methylobacterium sp. TC3–6	Coffee	+	−	+	−	+	+	+
Methylobacterium sp. TC3–7	Coffee	+	−	+	−	+	+	+
M. fujisawaense F5	Sugarcane	+	−	+	−	+	+	+
Methylobacterium sp. F7	Sugarcane	+	−	+	+	+	+	+
Methylobacterium sp. F8	Sugarcane	+	−	−	−	+	+	+
Methylobacterium sp. F9	Sugarcane	+	+	+	−	+	+	+
M. mesophilicum SR1.6/6	Citrus	+	+	+	+	+	+	+
Methylobacterium sp. SR3/27	Citrus	+	−	−	−	+	+	+
M. mesophilicum PR1/3	Citrus	+	+	−	−	+	+	−
Methylobacterium sp. PR3/11	Citrus	+	+	+	+	+	+	−
Methylotrophyc bacteria TP7	Sweet peper	+	+	+	+	+	+	+
M. hispanicum TP8	Sweet peper	+	−	+	−	+	+	+
Methylobacterium spp. R3E	Eucalyptus spp.	+	−	−	−	−	+	+
Methylobacterium spp. MA2.9	Laguncularia racemosa	+	+	+	−	+	+	+
Methylobacterium spp. MA3.1	Aviscenia shaueriana	+	+	−	+	+	+	+
Methylobacterium spp. MB1.1	Rhizophora mangle	+	−	+	−	+	−	+
Methylobacterium spp. MB1.3	Rhizophora mangle	+	−	−	−	−	−	−
Rhizobium SP	Laboratory collection	−	+	−	−	−	−	−
Sinorhizobium SP	Laboratory collection	−	−	−	−	−	−	−
Bacillus cereus	Laboratory collection	−	−	−	−	−	−	−
Total of 20		17	8	11	5	15	15	14