Figure 5.

Interaction interface between Fab 1F4 and DENV1 E protein.

1. Open-book representation showing the electrostatic potential of the interaction interface on the E protein (left) and the Fab 1F4 (right). The blue and red colors indicate positive and negative charges, respectively, whereas the white color shows neutral charge. The dotted lines indicate the border of the footprint between heavy and light chains on the E protein epitope (left) and also the corresponding border on the antibody paratope (right). Residues on E protein identified in the neutralization escape mutants (K47 and G274) are indicated, together with other residues that have opposite charges in other serotypes. The corresponding interacting residues on the Fab 1F4 light and heavy chains are indicated with cyan and green colored fonts, respectively.
2. Table of the list of putative interactions between DENV1 and Fab 1F4.