Figure 2.

NfnB-mediated reduction of BTZ043 and PBTZ169. Compounds (50 μM) were incubated with NfnB (6 μM) as described in Materials and Methods. Samples (10 μL) were taken at different times during the reaction and directly analyzed by LC-MS (ESI+). Results were analyzed by extracting the ion counts for the species of interest for each compound, namely the protonated forms of the original compound, and its nitroso, hydroxylamino and amino forms, as well as the azoxy adducts.