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. 2014 Feb 5;6(3):372–383. doi: 10.1002/emmm.201303575

Figure 4.

Figure 4

Treatment of M. marinum infected zebrafish embryos with BTZ.

A One hour post infection (1 hpi) zebrafish embryos that had been infected with 50-200 CFU of fluorescent M. marinum were treated for 5 days with BTZ043 or PBTZ169 at the concentrations indicated. Bacterial burden was assessed by fluorescence microscopy using a Leica MZ16FA microscope and customized software to quantify infection levels. Graphs represent data from 2 - 8 independent experiments: ns, not significant; **P < 0.001; ***P < 0.0001; one-way ANOVA, Bonferroni's multiple comparison test. The M strain was used for infection.

B–F Images of infected zebrafish embryos treated with PBTZ169 at 0 (WT), 5, 25 and 50 nM, or BTZ043 at 50 nM. The arrows indicate clusters of mycobacteria that were not present in fish treated with (P)BTZ at concentrations >25 nM. Note that the red fluorescence on the yolk/gut of the embryos is background staining.