Figure 5.

Effect of PBTZ169 treatment on infected zebrafish embryos. Zebrafish embryos were infected with either M. marinum strain M (A, B) or E11 (C, D). Treatment with PBTZ169 (25 μM) started at 0, 1 or 2 days post-infection (dpi) up to 5 dpi, giving exposure times of 5, 4 and 3 days, respectively. The bacterial burden was assessed by fluorescence microscopy and customized software to quantify the number of fluorescent pixels (A and C), and by enumeration of the CFU in lysed embryos (B and D), immediately after imaging was performed. In B and D, each data point represents the mean number of CFU per embryo of 3 embryo extracts plated, the bars indicate means and s.e.m. after log transformation. The graphs represent data from 2 to 4 independent experiments. ***P < 0.0001, one-way ANOVA, Bonferroni's multiple comparison test.

A,B Zebrafish embryos infected with M. marinum strain M.

C,D Zebrafish embryos infected with M. marinum strain E11.