Figure 5.

Rescue of SIL1-RNAi-induced migration defects.

1. Rescue of migration defects by an RNAi-resistant version of SIL1 (hSIL1) but not MSS-causing mutants. pCAG-EGFP was coelectroporated with pSUPER vector only (a) or pSUPER-mSIL1#1 together with pCAG vector (b), pCAG-Flag-hSIL1 (c), -SIL1-7G (d), -SIL1-L457P (e) or -SIL1-15del (f) into cerebral cortices at E14, followed by fixation at P0. Coronal sections were stained for GFP (white) and nuclei (blue). Dotted lines represent the pial and ventricular surfaces. Bar, 100 μm.
2. Quantification of the distribution of neurons in distinct regions of the cerebral cortex for each condition in (A, a–c). Error bars indicate s.d. (n = 3); **P = 0.0019 (layers II–IV), **P = 0.0016 (layers V–VI), **P = 0.0019 (SVZ/VZ; control versus SIL1#1), **P = 0.0041 (SVZ/VZ; SIL1#1 versus SIL1#1+hSIL1), ***P = 0.0005 (layers II–IV), ***P = 0.0005 (layers V–VI) by Fisher's LSD.
3. Quantification of the distribution of neurons in distinct regions of the cerebral cortex for each condition in (A, c–f). Error bars indicate s.d. (n = 3); *P = 0.012 (layers II–IV), *P = 0.0164 (layers V–VI), **P = 0.005 (layers II–IV; 7G), **P = 0.0012 (layers II–IV; L457P), **P = 0.0059 (layers V–VI; 7G), **P = 0.0059 (layers V–VI; L457P), **P = 0.0056 (layers SVZ/VZ) by Fisher's LSD.
4. Expression of hSIL1 and the mutants in dissociated cortical neurons. Cortical neurons (E17) were electroporated with pSUPER-SIL1#1 only (a) or together with pCAG-Flag vector (b), pCAG-Flag-hSIL1 (c), -SIL1-7G (d), -SIL1-L457P (e) or -SIL1-15del (f), and cultured in vitro for 48 h. Cells were then subjected to SDS-PAGE (20 μg protein per lane) followed by western blotting with anti-Flag M2, anti-HSPA5 or anti-β-tubulin. Asterisks indicate M2-positive bands.

Source data are available for this figure.