Figure 6. RNAi screen candidates interact genetically with RAS/MAPK pathway components.

(A–J) The Ras^V12 rough eye phenotype is dominantly suppressed by heterozygous mutations in Cka, gfzf, CG1603, Fip1, Prp19, Caper, and a trans-heterozygous mutation in CG4936. Fly eyes of the indicated genotypes were imaged by stereomicroscopy. The mapk alleles mapk^E1171 and rl¹ are used as positive controls. All fly eye images are from female flies except CG4936^DG10305/CG4936^EY10172, which is from a male fly; the rough eye phenotype was observed to be similar in males and females except in this case where males displayed a stronger genetic interaction. (K–N) Genetic interactions with rl¹ wing vein deletion phenotypes. rl¹/rl¹ flies display a slight deletion of the mid-section of the L4 wing vein that is not fully penetrant. The L4 deletion is enhanced, sometimes extending to the posterior cross vein (pcv) in Prp19^CE162 and Caper^f07714 heterozygous backgrounds (pictures shown served to illustrate detailed scoring results in Figure S8H). (O–R) Genetic interactions with rl¹ rough-eye phenotypes. The weak rough eye phenotype observed in rl¹ homozygotes is shown. The severity of this phenotype is increased in heterozygous mutant backgrounds for Prp19 and Caper; these flies display a further decrease in eye size and an increased eye roughness.