Skip to main content
. 2014 Mar 18;12(3):e1001813. doi: 10.1371/journal.pbio.1001813

Figure 5. Transcriptional activity of the GR mutants.

Figure 5

(A–B) Cos-7 cells were co-transfected with pEGFP-GR vectors and the MMTV-Luciferase reporter vector (A) or pkB-Luciferase reporter and pRelA expression vectors (B). Cells were incubated in the presence or absence of 100 nM Dex and luciferase activity was measured. (A) Values (induction efficiency) were expressed as percentage activity relative to Dex-treated wild-type GR. (B) Values (GR's inhibition efficiency on NF-κB activity) were expressed as percent induction relative to the control. (C) Nascent mRNA levels on MEFs cell lines stably expressing the GFPGR mutants. Means ± SEM from three independent experiments are shown. Bars with different superscript letters are significantly different from each other (p<0.05).