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. 2014 Mar 18;9(3):e89939. doi: 10.1371/journal.pone.0089939

Table 2. Flow cytometry analysis of Leishmania infantum promastigotes treated with betulin and betulinic acid derivatives (BT06, AB13) showing the percentage of propidium iodide (PI) and annexin-V positive cells.

Leishmania infantum intracellular entities (% of cells)
Annexin-V PI Annexine/PI
2 h 4 h 6 h 24 h 2 h 4 h 6 h 24 h 2 h 4 h 6 h 24 h
Control (BT06) 3.8 2 1.6 1.5 0.6 1.2 0.7 0.9 0.7 0.4 0.4 0.6
BT06 1.2 2.5 2.6 1.5 0.5 1.4 0.9 0.5 0.5 0.9 0.6 0.2
Control (AB13) 18.8 6.3 3.3 2.4 0.4 0.3 0 0.4 3.7 1.5 0.4 0.7
AB13 17.7 3.4 4.5 2.6 0.1 0 0.2 0 1.9 0.1 0.9 0.6