Table 2. Flow cytometry analysis of Leishmania infantum promastigotes treated with betulin and betulinic acid derivatives (BT06, AB13) showing the percentage of propidium iodide (PI) and annexin-V positive cells.
Leishmania infantum intracellular entities (% of cells) | ||||||||||||
Annexin-V | PI | Annexine/PI | ||||||||||
2 h | 4 h | 6 h | 24 h | 2 h | 4 h | 6 h | 24 h | 2 h | 4 h | 6 h | 24 h | |
Control (BT06) | 3.8 | 2 | 1.6 | 1.5 | 0.6 | 1.2 | 0.7 | 0.9 | 0.7 | 0.4 | 0.4 | 0.6 |
BT06 | 1.2 | 2.5 | 2.6 | 1.5 | 0.5 | 1.4 | 0.9 | 0.5 | 0.5 | 0.9 | 0.6 | 0.2 |
Control (AB13) | 18.8 | 6.3 | 3.3 | 2.4 | 0.4 | 0.3 | 0 | 0.4 | 3.7 | 1.5 | 0.4 | 0.7 |
AB13 | 17.7 | 3.4 | 4.5 | 2.6 | 0.1 | 0 | 0.2 | 0 | 1.9 | 0.1 | 0.9 | 0.6 |