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. 2014 Mar 18;9(3):e91703. doi: 10.1371/journal.pone.0091703

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© 2014 Spira et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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bacteria grown overnight in LB broth were transferred to HEp-2 cells monolayers in DMEM supplemented with 2% FBS and incubated for 3 h. The cell wells were washed and the bacteria were released, diluted and plated on LB agar for CFU counting. To the wild-type strain was attributed the value of 100%. Each column represents the mean standard error of the mean (S.E.M.) of three independent experiments.

Inline graphic — bacteria grown overnight in LB broth were transferred to HEp-2 cells monolayers in DMEM supplemented with 2% FBS and incubated for 3 h. The cell wells were washed and the bacteria were released, diluted and plated on LB agar for CFU counting. To the wild-type strain was attributed the value of 100%. Each column represents the mean standard error of the mean (S.E.M.) of three independent experiments.