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Bacteria grown overnight in LB broth were resuspended in DMEM and grown up to the mid-exponential phase. At this point the bacteria were harvested and immunoblotted with anti-SPA (FLAG 3X) antibodies. LRT9, wild-type strain; ΔrelA, LRT9 ΔrelA::Cm; prelA + ΔrelA, pLG19 transformed in ΔrelA::Cm; C, control strain - LRT9 not carrying a bfpA::SPA or eae::SPA fusion. The picture is representative of two independent experiments.
