| BS230 |
BW25113 ΔrelA::Cm |
This study |
| BW25113 |
E. coli K-12 carrying the recombineering plasmid pKD46 |
[30]
|
| CF1652 |
MG1655 relA::KmR |
M. Cashel |
| CF12489 |
argA::Tn10 relA
+
|
M. Cashel |
| CP01 |
MG1655 lacZ::Tn5
|
lab collection |
| KM32 |
recBCD::Ptac-gam-bet-exo cat
|
[54]
|
| LRT9 |
EPEC O111:abH2 |
[33]
|
| BS1230 |
LRT9 bfpA::SPA-Km |
This study |
| BS1264 |
GMF204 bfpA::SPA-Km |
This study |
| BS1298 |
LRT9 eae::SPA-Km |
This study |
| BS1299 |
GMF204 eae::SPA-Km |
This study |
| GMF204 |
LRT9 ΔrelA::KmR |
This study |
| GMF302 |
LRT9 argA::Tn10 relA
+
|
This study |
| LG01 |
LRT9 lacZ::Tn5
|
This study |
|
Plasmids
|
| pACT3 |
Cloning vector |
[55]
|
| pBS32 |
pLG19 GmR |
This study |
| pGM17 |
per promoter cloned upstream to the promoterless lacZ
|
[33]
|
| pJL148 |
SPA tag source |
[31]
|
| pKNOCK-Gm |
Plasmid carrying the gentamycin resistance (GmR) gene |
[56]
|
| pLG19 |
relA
+ cloned in plasmid pACT3 under Ptac
|
This study |
| pTZ57R/T |
Cloning vector |
Thermo |
|
Oligos
|
| bfpA813(F)-SPA |
AAATACTGATTCAACCAATAAAGTTACATATTTTATGAAGTCCATGGAAAAGAGAAG
|
| bfpA813(F)-SPATR |
CCCATATAATACGCCCAAAACAGGGCGTATTATGTAGATTCATATGAATATCCTCCTTAG
|
| eae-lrt9(F)-SPA |
TGGAGTAAACAATAAGAATGCTTTTTCTGTTTGTGTAAAATCCATGGAAAAGAGAAGA
|
| eae-lrt9(R)-SPA |
AATGAATTTTATTTTCCGGGATTTGAGATGTAATTAAATTCATATGAATATCCTCCTTAG
|
| relA1-fw-P1-fw |
ATGGTTGCGGTAAGAAGTGCACATATCAATAAGGCTGGTGAAGTGTAGGCTGGAGCTGCTTC
|
| relA1-rev-P1-rev |
CTAACTCCCGTGCAACCGACGCGCGTCGATAACATCCGGCACCATATGAATATCCTCCTTA
|
| relA6F |
ATCCACCAGGTCAATCTTCAC
|
| relA2633R |
AGGATATACCATTGCGCGAC
|
