Skip to main content
. 2014 Mar 18;9(3):e91752. doi: 10.1371/journal.pone.0091752

Figure 3. Expression of POR gene and protein in transfected HL60 cells.

Figure 3

HL60 cells were transfected with plasmids containing either control shRNA (CtrA) or POR shRNA and then (A) the expression of POR mRNA was tested in Real Time PCR. The bar charts show mean values (±SEM) of fold changes in mRNA levels relative to GAPDH mRNA levels. The control sample was calculated as 1. (B) The expression of POR protein in mitochondria of the cells exposed to 1,25D (10 nM) or ATRA (1 μM) for 96 h was detected using Western blot. Mitochondrial proteins were analyzed using anti-POR and anti-actin antibodies. Optical densities (OD) of POR bands divided by ODs of respective actin bands were calculated from 4 blots and mean values are given below the blots.