Table 1. Responses of AML patient's mononuclear cells to 1,25D or ATRA.

Patient			Differentiation	CYP24A1 expression	POR expression
No	AML subtype	Fusion gene/oncogene	increased CD14	1,25D-induced	1,25D-induced	ATRA-induced
9/2012	M4	FLT3-ITD	+++	14 100 x	5.7 x	2.5 x
12/2012	M1	-	-	41 900 x	2.6 x	1.7 x
13/2012	M1	-	+	14 700 x	1.7 x	downregulation
3/2013	M5b	FLT3-ITD	+++	8 720 x	1.4 x	downregulation
4/2013	M4	CBFB-MYH11	++	24 620 x	58 x	downregulation

Cells from peripheral blood of AML patients were exposed for 96 h to 10 nM 1,25D or to 1 μM ATRA. Then the expression of CD14 antigen in 1,25D-treated blast cells was studied in flow cytometry. From remaining 1,25D-treated blasts and from ATRA-treated blasts mRNA was isolated and the expression of CYP24A1 and POR genes was studied in Real Time PCR.