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. 2014 Mar 18;9(3):e91706. doi: 10.1371/journal.pone.0091706

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© 2014 Lauer et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Figure 1 A shows the survival of B. melitensis 16 M in Mono Mac 6 cells over 96 hours at 37°C. Figure 1 B depicts the internalization of rough and smooth B. melitensis and B. abortus strains into Mono Mac 6 cells at 37°C for up to 20 minutes. B. abortus strains appear to enter Mono Mac 6 cells slightly faster (within about 1 minute) than B. melitensis strains (within about 4 minutes) as indicated by the lowest CFU numbers which represent the time when all bacteria have become intracellular (extracellular bacteria have been killed by gentamicin and intracellular bacteria are not multiplying yet). The confocal images in Figures 1 C and D show the adherence of RFP-expressing B. melitensis to Mono Mac 6 cells at 1 minute (C) and 2 minutes (D) upon exposure at 4°C. Mono Mac 6 cells not exposed to Brucella are shown in Figure 1 E.