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Multiplex PCR characterization of GSTT1-1 in CCRF-CEM cells. GSTT1-1 was co-amplified with GSTM1 and beta globin as an internal positive control. Lanes A through E are CCRF-CEM cells at 50.5, 204, 408, 816, and 74 ng DNA, respectively. Lanes F and G are external controls (320 ng DNA) with lane F showing a subject positive for both GSTT1-1 and GSTM1 with lane G showing a subject null for both enzymes. DNA marker is a 50-bp ladder (Invitrogen).
