Figure 1. Functional output of cAMP, β-arrestin, ligand binding on membranes and live cells in response to calcitonins.

Head-to-head comparison of dose dependent cAMP accumulation (A) or β-arrestin recruitment (B), mediated by sCT and hCT after one (cAMP assay) and two (β-arrestin assay) hours of stimulation, respectively. The cAMP experiment was conducted with 100 μM IBMX in the medium to assess total cAMP production. Homologous competitive binding (C) and Ligand association (D) profiles of sCT and hCT using U2OS CALCR membranes preparations. Homologous competitive binding (E) and association (F) profiles of sCT and hCT using U2OS CALCR cells in culture. Data are shown as mean ± SEM and representative of three individual conducted experiments with six replicates (A,B) or three replicates (C–F).