. Author manuscript; available in PMC: 2014 Mar 20.

Published in final edited form as: Methods Mol Biol. 2012;907:109–122. doi: 10.1007/978-1-61779-974-7_6

Table 1.

Oligonucleotides^a

Name	Sequence (5′ -> 3′)
M13.rev	GAGCGGATAACAATTTCACACAGG
M13.uni	AGGGTTTTCCCAGTCACGACGTT
VH.FR4.for	TGGGGCAGAGGCACCCT
VL.FR3.rev	GCAGTAATAATCAGCCTCGTCC
H3.rev ^b	AGGGTGCCTCTGCCCCA (MNN)_5-20 TCTCACACAGTAATAAACAGCCG
L3.for ^b	GGACGAGGCTGATTATTACTGC (NNK)_9-11 TTCGGCGGAGGGACCAAG

The Tm of the oligonucleotides are all between 60 and 70 °C.

Design of the degenerated oligonucleotides is explained in Fig. 1 (see ^{Note 2}).