Table 2. Conservation of functional domains in ENS-1 like copies.
Protein size | Domains conservation and their position (AA) | ||||
kDa | HP1box (1) | cc-domain (2) | Sumo site 1 (3) | Sumo site 2 (4) | |
Sop.12 | 54 | 469 | 92-126 | 180 | 96 |
Sop.7 | 54 | 469 | 92-126 | 180 | 96 |
Sop.32 | 54 | 467 | 92-124 | 178 | 96 |
Sop.62 | 54 | 466 | 92-123 | 177 | 93 |
Sop.10 | 54 | 469 | 92-126 | 180 | 96 |
Sop.31 | 47 | / | 109-144 | 198 | 114 |
Sop.38 | 43 | / | 96-123 | 180 | 96 |
Sop.41 | 52 | / | 92-126 | 180 | 96 |
Sop.46 | 69 | / | 92-126 | 180 | 96 |
Sop.52 | 34 | / | 95-123 | 180 | 96 |
Sop.30 ( = ENS-3) | 77 | / | 422-450 | 507 | 423 |
Sop.18 | 54 | 469 | 92-126 | 180 | 96 |
Sop.2 | 54 | 469 | 92-126 | 180 | 96 |
Sop.69 | 29 | / | 92-124 | 180 | 96 |
Sop.54 | 18 | / | 85-124 | / | 92 |
Sop.15 | 23 | / | / | 36 | / |
Sop.34 | 23 | / | / | 77 | / |
Sop.26 | 42 | 357 | / | 68 | / |
Sop.24 | 32 | 272 | / | / | / |
Sop.40 | 20 | 161 | / | / | / |
Sop.8 | 20 | 161 | / | / | / |
Sop.22 | 20 | 161 | / | / | / |
Sop.35 | 20 | 161 | / | / | / |
Sop.20 | 20 | 161 | / | / | / |
Sop.3 | N/A | 113 | / | / | / |
In red are indicated the copies with an intact 3807 sequence and with an intact promoter.
(1) Based on the HP1 box motif involved in the interaction of ENS-1 with HP1γ: GLPTVRLE.
(2) Coiled-coil(cc) domain defined using the MultiCoil program.
(3) Sequence motif PLIKTEY.
(4) Sequence motif ESVKVTQ, inside the coiled-coil domain.