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. 2014 Mar 18;9(3):e91921. doi: 10.1371/journal.pone.0091921

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A–C) Primary hepatocyte cultures were transduced with adenovirus containing YFP-tagged ABCB11 on day 3. Fluorescence recovery after photobleaching (FRAP) was studied 3–5 days after transduction. A segment of the canalicular membrane was photobleached on the basis of YFP fluorescence, which was continuously monitored by confocal imaging. Elapsed times after photobleaching are indicated on the top of the pseudocolored images. Scale bar 10 µm. (D) Raw kinetics of fluorescence recovery measured in the photobleached membrane segment. (E) Fluorescence recovery curve was double normalized, and only the postbleach period was considered for kinetic analysis. The obtained data points were fitted as described in the text.