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. 2014 Mar 18;9(3):e91921. doi: 10.1371/journal.pone.0091921

Figure 3. Taurocholate stimulates canalicular trafficking of ABCB11.

Figure 3

(A) FRAP experiments with mouse hepatocytes expressing ABCB11-YFP were performed as described in Fig. 2. Pretreatment with 100 µM taurocholate (TC) substantially accelerated the fluorescence recovery (▪) as compared to untreated control cells (•). Nocodazole (Noc, 5 µg/ml) pretreatment blocked the second phase of biphasic fluorescence recovery curves, while the first, saturating phase was not affected (□, ○). Conclusively, the first phase presumably corresponds to lateral diffusion, whereas the second one reflects microtubule-dependent trafficking of ABCB11. (B) Kinetic parameters were determined by fitting the experimental points (see equation in the text). Only parameter B was significantly different between untreated and nocodazole-treated cells (p<0.05). Means ± S.E.M. of at least three independent experiments are shown.