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. 2014 Mar 18;9(3):e91921. doi: 10.1371/journal.pone.0091921

Figure 6. Kinetic analysis of intracellular particle movements.

Figure 6

Several time lapse image series of ABCB11-YFP-transduced hepatocytes similar to that shown in Videos S3, S4, S5 were acquired. A large number of ABCB11-YFP-containing intracellular vesicles were tracked by custom-written algorithm (see detail in Methods section), and their movements were analyzed (n>36). Distribution of average velocity (A–C), instantaneous velocity (D–F), and mean square displacement (G–I) are indicated for untreated control (A, D, G), untreated LKB1 −/− (B, E, H), and LKB1 −/− cells pretreated with 200 µM 8-Br-cAMP (C, F, I). Particle movements, especially directed motions are disrupted in LKB1 −/− hepatocytes, which can be restored by addition of cAMP.