Table 1. Binding properties as well as cellular and pore-forming activities of wild-type and LukS-PV mutants¹.

Mutation	Ki±95% (pM) ± confidence interval	Relative affinity to LukS-PV	Ca2+ entry slope (% of max/s) ± SEM	Ethidium entry (30 min) (% of control) ± SEM
LukS-PV	26±1.1	-	1.4±0.05	100
	(120±23)	-
T244A 2	6,190±560	238	0.50±0.093	25±5.9
	(11,000±2,000)	(92)
R73A 2	5,080±1,000	195	0.66±0.065	41±11
	(12,000±5,200)	(100)
Y250A 2	2,970±370	114	0.77±0.17	46±14
	(5,700±680)	(48)
Y184A 2	1,800±110	69	0.31±0.056	11±4.3
	(4,100±470)	(34)
H245A 2	1,800±260	69	1.1±0.059	80±6.7
	(4,200±900)	(35)
R242A	619±92	24	1.2±0.076	83±4.3
F260A	427±30	16	1.2±0.11	88±2.2
Y246A	365±47	14	1.0±0.098	80±6.7
Y181A	155±12	6	0.95± 0.045	54±5.2
N248A	74±8.2	2.8	0.93±0.078	77±7.6
K182A	68±5.2	2.6	1.0±0.062	79±3.4
H257A	56±4.5	2.2	1.3±0.12	96±5.5
H173A	45±1.3	1.7	1.2±0.073	73±5.8
D190A	44±2.6	1.7	1.3±0.04	90±3
Q186A	43±0.67	1.7	1.1±0.072	80±4.4
Y191A	41±2.9	1.6	1.9±0.16	111±2.4
R263A	38±1.3	1.5	1.4±0.068	90±2.8

¹ K_i values, calcium entry slope and ethidium entry at 30 min were obtained for wild-type LukS-PV and all mutants with hPMNs. K_i values with U937-C5aR cells were obtained for wild-type LukS-PV and for the most affected mutants (values given in parenthesis).

²Mutations causing a significant decrease in LukS-PV affinity for hPMNs (p<0.001, one-way ANOVA with Dunnett's post test).