Figure 2. Diagram of serial deletions of Pou4f2 and Isl1.

Diagram of the Pou4f2 (Left) and Isl1 (right) protein structures and the truncated deletions we expressed in E. Coli. The amino acid residues for each deletion and their estimated molecular weights are indicated. All proteins are His-tagged at the C terminus for easy detection by Western Blotting, although the Isl1 protein and its truncates are detected by a polyclonal rabbit anti-Isl1. * indicates constructs capable of protein-protein interaction (see Figs. 3 5 ).