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. 2014 Mar 18;9(3):e92113. doi: 10.1371/journal.pone.0092113

Figure 5. Comparison of the catecholaminergic inducing activities of five different bHLH factors.

Figure 5

(A) Whole mount in situ hybridization analysis of Tyrosine Hydroxylase (TH) expression on stage 24 embryos injected with the indicated mRNAs in one blastomere at the two-cell stage (anterior on the left, dorsal side up). Arrowheads indicate the position of previously described TH-positive antero-ventral neurons [53], while arrows point to ectopic TH staining. Note that, among all tested bHLH factors, only Ascl1 induces ectopic TH expression. Sibling embryos were also hybridized with the tubb2b probe as a positive control (see Figure 3A). (B) Quantification of embryos displaying ectopic TH+ neurons, showing that Neurog2 and Ptf1a significantly reduce the catecholaminergic inducing activity of Ascl1. Total number of analyzed embryos per condition is indicated in each bar. Error bars represent 95% confidence intervals. p<0,05 (*) (binomial test). Scale bar represents 500 μm.